|
KMID : 0043319970200030247
|
|
Archives of Pharmacal Research
1997 Volume.20 No. 3 p.247 ~ p.252
|
|
|
Determination of oestrone, 17α-and 17β-oestradiol in bovine aqueous humor using gas chromatography-negative ion chemical ionization mass spectrometry
|
|
Zafar-Iqbal
Midgley John-M
Watson David-G
|
|
|
Abstract
|
|
|
|
Perfluorotolyl (PFT)-ether and perfluorotoly-trimethylsilyl (PFT-TMS) ether derivatives of oestrone, - and oestradiol were prepared under phase transfer conditons. The former derivatives under negative ion chemical ionization conditions gave significant ions in the mass spectrometer but - and -oestradiol gave poor resolution. However, the PFT-TMS derivatives of 17.- and-oestradiol showed good resolution. These derivatives were used for the analysis of oestrogens in bovine aqueous humour, vitreous humour and retina. The mean concentrations of oestrone in bovine aqueous humour (n=18), vitreous humour (n=18) and bovine retina (n=4) were , and , respectively. -Oestradiol was detected in 16 out of 18 samples of bovine aqueous humour and vitreous humour and the mean concentrations were and , respectively. The mean concentration of 17.betha.-oestradiol in aqueous humour (n=7) and vitreous homour (n=11) and , respectively. In retina the concentrations of both steroids were below the detection limit.
|
|
|
KEYWORD
|
|
|
Oestrone, 17α-Oestradiol, 17β-Oestradial, GC-MS, Aqueous hummour, Vitreous humour, Retina
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|